In this work, the impacts of crystal orientation and applied uni-axial external tensile stress on the local hydrogen content of Ni, austenitic stainless steel and duplex stainless steel was studied. We characterized the changes of surface topography during in-situ charging and also quantified the hydrogen content at different crystal orientations and grain boundaries. The characterization was performed with the aid of various AFM-based techniques after exact classification of the crystal orientations and Grain boundaries via EBSD method. Furthermore, the contribution of the hydrogen on the local mechanical properties was evaluated using the in-situ nanoindentation technique. The information could give us the opportunity to classify various grain orientations and grain boundaries based on their sensitivity to hydrogen embrittlement and hence make a clear recipe for engineering of grain boundaries.